945 research outputs found
Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p
During mating, budding yeast cells reorient growth toward the highest concentration of pheromone. Bni1p, a formin homologue, is required for this polarized growth by facilitating cortical actin cable assembly. Fus3p, a pheromone-activated MAP kinase, is required for pheromone signaling and cell fusion. We show that Fus3p phosphorylates Bni1p in vitro, and phosphorylation of Bni1p in vivo during the pheromone response is dependent on Fus3p. fus3 mutants exhibited multiple phenotypes similar to bni1 mutants, including defects in actin and cell polarization, as well as Kar9p and cytoplasmic microtubule localization. Disruption of the interaction between Fus3p and the receptor-associated Gα subunit caused similar mutant phenotypes. After pheromone treatment, Bni1p-GFP and Spa2p failed to localize to the cortex of fus3 mutants, and cell wall growth became completely unpolarized. Bni1p overexpression suppressed the actin assembly, cell polarization, and cell fusion defects. These data suggest a model wherein activated Fus3p is recruited back to the cortex, where it activates Bni1p to promote polarization and cell fusion.</jats:p
3-D Tracking and Visualization of Hundreds of Pt-Co Fuel Cell Nanocatalysts During Electrochemical Aging
We present an electron tomography method that allows for the identification
of hundreds of electrocatalyst nanoparticles with one-to-one correspondence
before and after electrochemical aging. This method allows us to track, in
three-dimensions (3-D), the trajectories and morphologies of each Pt-Co
nanocatalyst on a fuel cell carbon support. The use of atomic-scale electron
energy loss spectroscopic imaging enables the correlation of performance
degradation of the catalyst with changes in particle/inter-particle
morphologies, particle-support interactions and the near-surface chemical
composition. We found that, aging of the catalysts under normal fuel cell
operating conditions (potential scans from +0.6 V to +1.0 V for 30,000 cycles)
gives rise to coarsening of the nanoparticles, mainly through coalescence,
which in turn leads to the loss of performance. The observed coalescence events
were found to be the result of nanoparticle migration on the carbon support
during potential cycling. This method provides detailed insights into how
nanocatalyst degradation occurs in proton exchange membrane fuel cells
(PEMFCs), and suggests that minimization of particle movement can potentially
slow down the coarsening of the particles, and the corresponding performance
degradation.Comment: Nano Letters, accepte
Mapping local optical densities of states in silicon photonic structures with nanoscale electron spectroscopy
Relativistic electrons in a structured medium generate radiative losses such
as Cherenkov and transition radiation that act as a virtual light source,
coupling to the photonic densities of states. The effect is most pronounced
when the imaginary part of the dielectric function is zero, a regime where in a
non-retarded treatment no loss or coupling can occur. Maps of the resultant
energy losses as a sub-5nm electron probe scans across finite waveguide
structures reveal spatial distributions of optical modes in a spectral domain
ranging from near-infrared to far ultraviolet.Comment: 18 pages, 4 figure
Interacting electrons in disordered potentials: Conductance versus persistent currents
An expression for the conductance of interacting electrons in the diffusive
regime as a function of the ensemble averaged persistent current and the
compressibility of the system is presented. This expression involves only
ground-state properties of the system. The different dependencies of the
conductance and persistent current on the electron-electron interaction
strength becomes apparent. The conductance and persistent current of a small
system of interacting electrons are calculated numerically and their variation
with the strength of the interaction is compared. It is found that while the
persistent current is enhanced by interactions, the conductance is suppressed.Comment: REVTeX, 4 pages, 3 figures, all uuencoded, accepted for publication
in PR
Loss of a 20S Proteasome Activator in Saccharomyces cerevisiae Downregulates Genes Important for Genomic Integrity, Increases DNA Damage, and Selectively Sensitizes Cells to Agents With Diverse Mechanisms of Action
Cytoprotective functions of a 20S proteasome activator were investigated. Saccharomyces cerevisiae Blm10 and human 20S proteasome activator 200 (PA200) are homologs. Comparative genome-wide analyses of untreated diploid cells lacking Blm10 and growing at steady state at defined growth rates revealed downregulation of numerous genes required for accurate chromosome structure, assembly and repair, and upregulation of a specific subset of genes encoding protein-folding chaperones. Blm10 loss or truncation of the Ubp3/Blm3 deubiquitinating enzyme caused massive chromosomal damage and cell death in homozygous diploids after phleomycin treatments, indicating that Blm10 and Ubp3/Blm3 function to stabilize the genome and protect against cell death. Diploids lacking Blm10 also were sensitized to doxorubicin, hydroxyurea, 5-fluorouracil, rapamycin, hydrogen peroxide, methyl methanesulfonate, and calcofluor. Fluorescently tagged Blm10 localized in nuclei, with enhanced fluorescence after DNA replication. After DNA damage that caused a classic G2/M arrest, fluorescence remained diffuse, with evidence of nuclear fragmentation in some cells. Protective functions of Blm10 did not require the carboxyl-terminal region that makes close contact with 20S proteasomes, indicating that protection does not require this contact or the truncated Blm10 can interact with the proteasome apart from this region. Without its carboxyl-terminus, Blm10(−339aa) localized to nuclei in untreated, nonproliferating (G0) cells, but not during G1 S, G2, and M. The results indicate Blm10 functions in protective mechanisms that include the machinery that assures proper assembly of chromosomes. These essential guardian functions have implications for ubiquitin-independent targeting in anticancer therapy. Targeting Blm10/PA200 together with one or more of the upregulated chaperones or a conventional treatment could be efficacious
The Structure of IR Luminous Galaxies at 100 Microns
We have observed twenty two galaxies at 100 microns with the Kuiper Airborne
Observatory in order to determine the size of their FIR emitting regions. Most
of these galaxies are luminous far-infrared sources, with L_FIR > 10^11 L_sun.
This data constitutes the highest spatial resolution ever achieved on luminous
galaxies in the far infrared. Our data includes direct measurements of the
spatial structure of the sources, in which we look for departures from point
source profiles. Additionally, comparison of our small beam 100 micron fluxes
with the large beam IRAS fluxes shows how much flux falls beyond our detectors
but within the IRAS beam. Several sources with point- like cores show evidence
for such a net flux deficit. We clearly resolved six of these galaxies at 100
microns and have some evidence for extension in seven others. Those galaxies
which we have resolved can have little of their 100 micron flux directly
emitted by a point-like active galactic nucleus (AGN). Dust heated to ~40 K by
recent bursts of non-nuclear star formation provides the best explanation for
their extreme FIR luminosity. In a few cases, heating of an extended region by
a compact central source is also a plausible option. Assuming the FIR emission
we see is from dust, we also use the sizes we derive to find the dust
temperatures and optical depths at 100 microns which we translate into an
effective visual extinction through the galaxy. Our work shows that studies of
the far infrared structure of luminous infrared galaxies is clearly within the
capabilities of new generation far infrared instrumentation, such as SOFIA and
SIRTF.Comment: 8 tables, 23 figure
Coherent Control of Isotope Separation in HD+ Photodissociation by Strong Fields
The photodissociation of the HD+ molecular ion in intense short- pulsed
linearly polarized laser fields is studied using a time- dependent wave-packet
approach where molecular rotation is fully included. We show that applying a
coherent superposition of the fundamental radiation with its second harmonic
can lead to asymmetries in the fragment angular distributions, with significant
differences between the hydrogen and deuterium distributions in the long
wavelength domain where the permanent dipole is most efficient. This effect is
used to induce an appreciable isotope separation.Comment: Physical Review Letters, 1995 (in press). 4 pages in revtex format, 3
uuencoded figures. Full postcript version available at:
http://chemphys.weizmann.ac.il/~charron/prl.ps or
ftp://scipion.ppm.u-psud.fr/coherent.control/prl.p
High-Velocity Clouds in the Nearby Spiral Galaxy M 83
We present deep HI 21-cm and optical observations of the face-on spiral
galaxy M 83 obtained as part of a project to search for high-velocity clouds
(HVCs) in nearby galaxies. Anomalous-velocity neutral gas is detected toward M
83, with 5.6x10^7 Msolar of HI contained in a disk rotating 40-50 km/s more
slowly in projection than the bulk of the gas. We interpret this as a
vertically extended thick disk of neutral material, containing 5.5% of the
total HI within the central 8 kpc. Using an automated source detection
algorithm to search for small-scale HI emission features, we find eight
distinct, anomalous-velocity HI clouds with masses ranging from 7x10^5 to
1.5x10^7 Msolar and velocities differing by up to 200 km/s compared to the HI
disk. Large on-disk structures are coincident with the optical spiral arms,
while unresolved off-disk clouds contain no diffuse optical emission down to a
limit of 27 r' mag per square arcsec. The diversity of the thick HI disk and
larger clouds suggests the influence of multiple formation mechanisms, with a
galactic fountain responsible for the slowly-rotating disk and on-disk discrete
clouds, and tidal effects responsible for off-disk cloud production. The mass
and kinetic energy of the HI clouds are consistent with the mass exchange rate
predicted by the galactic fountain model. If the HVC population in M 83 is
similar to that in our own Galaxy, then the Galactic HVCs must be distributed
within a radius of less than 25 kpc.Comment: 30 pages, 23 figures; accepted for publication in ApJ. Some figures
have been altered to reduce their siz
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